Hotspot

ycf3-trnS(GGA)

LSC · 45020-45867 · 848 bp

Back to Paulownia

Pi 0.00371
MarkerSeek score 52.60
Primer available yes
Label ycf3-trnS(GGA)

Feature Metadata

label_name
ycf3-trnS(GGA)
feature_type
igs
region
LSC
length_bp
848
variable_sites
8
pi
0.00371
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS(GGA)_p1 TGCCTCCTTTTCTCCTGAAG CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1017-1020 bp 1.0 73.2
ycf3-trnS(GGA)_p2 GCCTCCTTTTCTCCTGAAGT CCCTCGGTAAACAAAAGCCT 50.0 50.0 57.1 58.1 1016-1019 bp 1.0 73.2
ycf3-trnS(GGA)_p3 TGCCTCCTTTTCTCCTGAAG ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.1 58.2 1038-1041 bp 1.0 72.7
ycf3-trnS(GGA)_p4 GCCTCCTTTTCTCCTGAAGT ACGGAAAGAGAGGGATTCGA 50.0 50.0 57.1 58.2 1037-1040 bp 1.0 72.7
ycf3-trnS(GGA)_p5 TGCCTCCTTTTCTCCTGAAG TTCCAATGCTACGCCTTGAA 50.0 45.0 57.1 57.8 988-991 bp 1.0 72.4