Hotspot

trnT(GGU)-psbD

LSC · 32101-33368 · 1268 bp

Back to Paulownia

Pi 0.0027

MarkerSeek score 47.30

Primer available yes

Label trnT(GGU)-psbD

Feature Metadata

label_name: trnT(GGU)-psbD
feature_type: igs
region: LSC
length_bp: 1268
variable_sites: 13
pi: 0.0027
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT(GGU)-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1427-1437 bp	1.0	79.4
trnT(GGU)-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1430-1440 bp	1.0	79.2
trnT(GGU)-psbD_p3	ACATAGTGACTCGTTCGGGA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.2	58.1	1475-1485 bp	1.0	78.5
trnT(GGU)-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1417-1427 bp	1.0	78.3
trnT(GGU)-psbD_p5	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1420-1430 bp	1.0	78.1