Hotspot

trnK(UUU)-rps16

LSC · 4447-5336 · 890 bp

Back to Paulownia

Pi 0.0135
MarkerSeek score 55.80
Primer available yes
Label trnK(UUU)-rps16

Feature Metadata

label_name
trnK(UUU)-rps16
feature_type
igs
region
LSC
length_bp
890
variable_sites
46
pi
0.0135
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK(UUU)-rps16_p1 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 1013-1019 bp 1.0 69.5
trnK(UUU)-rps16_p2 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 1012-1018 bp 1.0 69.5
trnK(UUU)-rps16_p3 AAAGCCGAGTACTCTACCGT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 1012-1018 bp 1.0 68.7
trnK(UUU)-rps16_p4 AAGCCGAGTACTCTACCGTT TGCTCAACCCACAGGAACT 50.0 52.6 58.2 58.8 1011-1017 bp 1.0 68.7
trnK(UUU)-rps16_p5 TCTAGCCGCACTTAAAAGCC GTGCTCAACCCACAGGAAC 50.0 57.9 58.3 58.7 1027-1033 bp 1.0 68.6