Hotspot

rpoA

LSC · 79146-80126 · 981 bp

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Pi 0.0566

MarkerSeek score 69.80

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 981
variable_sites: 111
pi: 0.0566
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TAATGGTTTCAGTACCCGCG	CGGTACTTGTGGATTCAGGG	50.0	55.0	58.0	58.0	1719-1749 bp	1.0	86.3
rpoA_p2	TAATGGTTTCAGTACCCGCG	CAGATGTACGGGGTCAAGTG	50.0	55.0	58.0	58.0	1756-1786 bp	1.0	86.2
rpoA_p3	TAATGGTTTCAGTACCCGCG	CAAGGTATGAAACGAGCCGA	50.0	50.0	58.0	58.0	1631-1661 bp	0.857143	80.6
rpoA_p4	TAATGGTTTCAGTACCCGCG	TCAAGGTATGAAACGAGCCG	50.0	50.0	58.0	58.0	1632-1662 bp	0.857143	80.6
rpoA_p5	TAATGGTTTCAGTACCCGCG	AAGGTATGAAACGAGCCGAC	50.0	50.0	58.0	58.0	1630-1660 bp	0.857143	80.6