Hotspot

ycf2-trnI-CAU

IRa · 151437-151599 · 163 bp

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Pi 0.102

MarkerSeek score 71.20

Primer available yes

Label ycf2-trnI-CAU

Feature Metadata

label_name: ycf2-trnI-CAU
feature_type: igs
region: IRa
length_bp: 163
variable_sites: 43
pi: 0.102
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnI-CAU_p1	GTTGAACCCTGTGAATTGCG	GTTGGGCGCTTTAACCATTC	50.0	50.0	57.9	58.0	400-615 bp	0.857143	83.0
ycf2-trnI-CAU_p2	GTTGAACCCTGTGAATTGCG	ATTGGCATGCATCCAGTAGG	50.0	50.0	57.9	58.0	449-664 bp	0.857143	82.5
ycf2-trnI-CAU_p3	TATCGTTTCTGGGCCCAATG	ATTGGCATGCATCCAGTAGG	50.0	50.0	57.9	58.0	604-819 bp	0.857143	81.5
ycf2-trnI-CAU_p4	ACTCCCTTTCGGATTCAGGA	GTTGGGCGCTTTAACCATTC	50.0	50.0	58.0	58.0	668-883 bp	0.857143	81.2
ycf2-trnI-CAU_p5	ACTCCCTTTCGGATTCAGGA	ATTGGCATGCATCCAGTAGG	50.0	50.0	58.0	58.0	717-932 bp	0.857143	81.0