Hotspot

ycf1-trnN-GUU

SSC · 128653-129108 · 456 bp

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Pi 0.0406

MarkerSeek score 65.20

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: SSC
length_bp: 456
variable_sites: 50
pi: 0.0406
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	GGTTCCTTCTTCCACAACGT	CCCCGTAACTCTTCTTCAGC	50.0	55.0	58.0	58.0	896-1363 bp	0.857143	83.3
ycf1-trnN-GUU_p2	GGTTCCTTCTTCCACAACGT	CCCGTAACTCTTCTTCAGCC	50.0	55.0	58.0	58.0	895-1362 bp	0.857143	83.2
ycf1-trnN-GUU_p3	GGTTCCTTCTTCCACAACGT	GAACTTGGGTGTGGGTCTTT	50.0	50.0	58.0	57.9	948-1448 bp	0.857143	82.6
ycf1-trnN-GUU_p4	GGTTCCTTCTTCCACAACGT	GGGTGTGGGTCTTTTGTCTT	50.0	50.0	58.0	57.9	942-1442 bp	0.857143	82.6
ycf1-trnN-GUU_p5	GGTTCCTTCTTCCACAACGT	GCCCCGTAACTCTTCTTCAG	50.0	55.0	58.0	58.0	1018-1364 bp	0.714286	75.8