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rps15-ycf1

SSC · 123379-123838 · 460 bp

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Pi 0.0391

MarkerSeek score 71.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 460
variable_sites: 39
pi: 0.0391
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GGTGCTGAGGACCCATATTG	CGTCGGTTTAGCTGGATGAA	55.0	50.0	58.0	58.0	1381-1452 bp	0.857143	80.7
rps15-ycf1_p2	GGTGCTGAGGACCCATATTG	TCCACTGCAAGCCATCAAAT	55.0	45.0	58.0	58.1	1252-1323 bp	0.857143	80.4
rps15-ycf1_p3	ATGAGTCGAAGAACACCGTG	CGTCGGTTTAGCTGGATGAA	50.0	50.0	57.9	58.0	1409-1480 bp	0.857143	80.3
rps15-ycf1_p4	TAGCTGGCCCTCTTAGGTAC	CGTCGGTTTAGCTGGATGAA	55.0	50.0	57.9	58.0	1622-1693 bp	0.857143	80.0
rps15-ycf1_p5	GGTGCTGAGGACCCATATTG	AATAACGTTGTGGCGCAAAG	55.0	45.0	58.0	58.0	1476-1656 bp	0.714286	74.1