Hotspot

trnI-CAU-ycf2

IRb · 87746-87907 · 162 bp

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Pi 0.125

MarkerSeek score 72.70

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: IRb
length_bp: 162
variable_sites: 56
pi: 0.125
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	GTTGAACCCTGTGAATTGCG	50.0	50.0	58.0	57.9	400-811 bp	1.0	89.3
trnI-CAU-ycf2_p2	ATTGGCATGCATCCAGTAGG	GTTGAACCCTGTGAATTGCG	50.0	50.0	58.0	57.9	449-860 bp	1.0	89.1
trnI-CAU-ycf2_p3	ATTGGCATGCATCCAGTAGG	TATCGTTTCTGGGCCCAATG	50.0	50.0	58.0	57.9	604-1015 bp	1.0	88.4
trnI-CAU-ycf2_p4	GTTGGGCGCTTTAACCATTC	ACTCCCTTTCGGATTCAGGA	50.0	50.0	58.0	58.0	668-1079 bp	1.0	87.6
trnI-CAU-ycf2_p5	ATTGGCATGCATCCAGTAGG	ACTCCCTTTCGGATTCAGGA	50.0	50.0	58.0	58.0	717-1128 bp	1.0	87.3