Hotspot

rpl23-trnI-CAU

IRb · 87502-87671 · 170 bp

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Pi 0.00336
MarkerSeek score 26.70
Primer available yes
Label rpl23-trnI-CAU

Feature Metadata

label_name
rpl23-trnI-CAU
feature_type
igs
region
IRb
length_bp
170
variable_sites
2
pi
0.00336
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
26.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl23-trnI-CAU_p1 CCGAATGCTTTTGTCTGCAG AGGTTCAATTCCTACTGGATGC 50.0 45.5 58.0 58.1 238 bp 1.0 63.6
rpl23-trnI-CAU_p2 CCGAATGCTTTTGTCTGCAG GGTTCAATTCCTACTGGATGC 50.0 47.6 58.0 56.7 237 bp 1.0 61.7
rpl23-trnI-CAU_p3 CCGAATGCTTTTGTCTGCAG GGTTCAATTCCTACTGGATGCA 50.0 45.5 58.0 58.4 237 bp 1.0 61.4
rpl23-trnI-CAU_p4 AACCGAATGCTTTTGTCTGC AGGTTCAATTCCTACTGGATGC 45.0 45.5 57.6 58.1 240 bp 1.0 60.4
rpl23-trnI-CAU_p5 AACCGAATGCTTTTGTCTGC GGTTCAATTCCTACTGGATGC 45.0 47.6 57.6 56.7 239 bp 1.0 58.6