Hotspot

petN-psbM

LSC · 30331-31403 · 1073 bp

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Pi 0.0322

MarkerSeek score 60.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1073
variable_sites: 50
pi: 0.0322
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGGAAGAAGTGGGCTCTAA	CGTAAAAACCATCAGTCAAAGTCA	55.0	37.5	58.7	57.9	873-1127 bp	1.0	50.0
petN-psbM_p2	GGGGAAGAAGTGGGCTCTAA	ACGTAAAAACCATCAGTCAAAGTC	55.0	37.5	58.7	57.9	874-1128 bp	1.0	50.0
petN-psbM_p3	GGGGAAGAAGTGGGCTCTAA	ACGTAAAAACCATCAGTCAAAGT	55.0	34.8	58.7	56.9	874-1128 bp	1.0	49.8
petN-psbM_p4	TATGGGGAAGAAGTGGGCTC	CGTAAAAACCATCAGTCAAAGTCA	55.0	37.5	58.8	57.9	876-1130 bp	1.0	49.4
petN-psbM_p5	TATGGGGAAGAAGTGGGCTC	ACGTAAAAACCATCAGTCAAAGTC	55.0	37.5	58.8	57.9	877-1131 bp	1.0	49.4