Hotspot

ycf2

LSC · 85924-92229 · 6306 bp

Back to Pastinaca

Pi 0.0117

MarkerSeek score 59.30

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6306
variable_sites: 74
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	GGCAAGCGGATCCTTTATGA	CGGAAACTAAGCTCCCACTC	50.0	55.0	58.0	58.0	893-899 bp	1.0	80.8
ycf2_p2	GTTCTTGCAGAGTGGAACCA	CAGACGGAAACTAAGCTCCC	50.0	55.0	58.0	58.0	844-850 bp	1.0	80.8
ycf2_p3	GTTCTTGCAGAGTGGAACCA	CCAGACGGAAACTAAGCTCC	50.0	55.0	58.0	58.0	845-851 bp	1.0	80.8
ycf2_p4	GTTCTTGCAGAGTGGAACCA	CCCAGACGGAAACTAAGCTC	50.0	55.0	58.0	58.0	846-852 bp	1.0	80.8
ycf2_p5	GTTCTTGCAGAGTGGAACCA	CGGAAACTAAGCTCCCACTC	50.0	55.0	58.0	58.0	840-846 bp	1.0	80.8