Hotspot

ycf1

SSC · 126227-131713 · 5487 bp

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Pi 0.00785

MarkerSeek score 44.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5487
variable_sites: 43
pi: 0.00785
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGGGAGTTCCATGATACTCA	CCCTTCCCTTTGGTTCTGTC	47.6	55.0	58.3	58.1	931 bp	1.0	70.4
ycf1_p2	ACGGGAGTTCCATGATACTCA	CCTTCCCTTTGGTTCTGTCC	47.6	55.0	58.3	58.1	930 bp	1.0	70.4
ycf1_p3	ACGGGAGTTCCATGATACTCA	CTTCCCTTTGGTTCTGTCCC	47.6	55.0	58.3	58.1	929 bp	1.0	70.4
ycf1_p4	ACGGGAGTTCCATGATACTCA	GCTGAGGACTTCGATGAGTG	47.6	55.0	58.3	58.1	1175 bp	1.0	70.3
ycf1_p5	ACGGGAGTTCCATGATACTCA	GAAACCTTGGCACAGATCCA	47.6	50.0	58.3	58.1	1038 bp	1.0	70.3