Hotspot

rps16

LSC · 3606-4696 · 1091 bp

Back to Pastinaca

Pi 0.0101

MarkerSeek score 64.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1091
variable_sites: 11
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCAATTTTGTTTGATTAGGACCA	TGTATCGACGGAACCAATGAC	30.4	47.6	54.7	58.1	1198-1199 bp	1.0	40.9
rps16_p2	TCAATTTTGTTTGATTAGGACCA	GTATCGACGGAACCAATGACT	30.4	47.6	54.7	57.8	1197-1198 bp	1.0	40.9
rps16_p3	TCAATTTTGTTTGATTAGGACCAAG	TGTATCGACGGAACCAATGAC	32.0	47.6	56.5	58.1	1198-1199 bp	1.0	40.9
rps16_p4	TCAATTTTGTTTGATTAGGACCA	TGTATCGACGGAACCAATGA	30.4	45.0	54.7	56.7	1198-1199 bp	1.0	40.9
rps16_p5	TCAATTTTGTTTGATTAGGACCAAG	GTATCGACGGAACCAATGACT	32.0	47.6	56.5	57.8	1197-1198 bp	1.0	40.9