Hotspot

petB

LSC · 74421-75819 · 1399 bp

Back to Pastinaca

Pi 0.00858

MarkerSeek score 63.70

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1399
variable_sites: 12
pi: 0.00858
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	TTGGTAGTTTGACCGCGAAA	TCCCCAGTGATAAAGGATGGA	45.0	47.6	58.0	57.9	1491 bp	1.0	71.1
petB_p2	TTTGGTAGTTTGACCGCGAA	TCCCCAGTGATAAAGGATGGA	45.0	47.6	58.0	57.9	1492 bp	1.0	71.1
petB_p3	TTTTGGTAGTTTGACCGCGA	TCCCCAGTGATAAAGGATGGA	45.0	47.6	58.0	57.9	1493 bp	1.0	71.1
petB_p4	TGGTAGTTTGACCGCGAAAT	TCCCCAGTGATAAAGGATGGA	45.0	47.6	57.8	57.9	1490 bp	1.0	70.1
petB_p5	TTGGTAGTTTGACCGCGAAA	CTCCCCAGTGATAAAGGATGG	45.0	52.4	58.0	57.5	1492 bp	1.0	68.3