Hotspot

trnL-CAA-trnH-GUG

IRa · 147017-148517 · 1501 bp

Back to Pastinaca

Pi 0.0172

MarkerSeek score 63.20

Primer available yes

Label trnL-CAA-trnH-GUG

Feature Metadata

label_name: trnL-CAA-trnH-GUG
feature_type: igs
region: IRa
length_bp: 1501
variable_sites: 25
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-trnH-GUG_p1	AAATGGTAGACACGCGAGAC	CTCAAGGCAGTGGATTGTGA	50.0	50.0	58.0	57.8	1576-1626 bp	1.0	82.2
trnL-CAA-trnH-GUG_p2	TGAAATGGTAGACACGCGAG	CTCAAGGCAGTGGATTGTGA	50.0	50.0	58.0	57.8	1578-1628 bp	1.0	82.2
trnL-CAA-trnH-GUG_p3	GCCTTGGTGGTGAAATGGTA	CTCAAGGCAGTGGATTGTGA	50.0	50.0	58.1	57.8	1588-1638 bp	1.0	81.5
trnL-CAA-trnH-GUG_p4	TGGTGAAATGGTAGACACGC	CTCAAGGCAGTGGATTGTGA	50.0	50.0	58.2	57.8	1581-1631 bp	1.0	80.6
trnL-CAA-trnH-GUG_p5	GAAATGGTAGACACGCGAGA	CTCAAGGCAGTGGATTGTGA	50.0	50.0	57.7	57.8	1577-1627 bp	1.0	80.0