Hotspot

rps8-rpl14

LSC · 80017-80213 · 197 bp

Back to Pastinaca

Pi 0.0255

MarkerSeek score 63.40

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 5
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TGCTATTCGAACCACCCTTT	GAAACCCCAAAGGAACTCGA	45.0	50.0	57.1	57.7	375-376 bp	1.0	72.2
rps8-rpl14_p2	TGCTATTCGAACCACCCTTT	GACCAAGAAGGAAACCCCAA	45.0	50.0	57.1	57.6	385-386 bp	1.0	71.3
rps8-rpl14_p3	TGCTATTCGAACCACCCTTT	TTGACCAAGAAGGAAACCCC	45.0	50.0	57.1	57.6	387-388 bp	1.0	71.3
rps8-rpl14_p4	TGCTATTCGAACCACCCTTT	ATTTTTGGTGCAATTGCCCG	45.0	45.0	57.1	58.5	355-356 bp	1.0	70.6
rps8-rpl14_p5	ATGCTATTCGAACCACCCTT	GAAACCCCAAAGGAACTCGA	45.0	50.0	56.9	57.7	376-377 bp	1.0	70.3