Hotspot

rpoB-trnC-GCA

LSC · 25682-26895 · 1214 bp

Back to Pastinaca

Pi 0.00663

MarkerSeek score 63.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1214
variable_sites: 8
pi: 0.00663
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCACGAACCTACAAAAGCCT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1351-1358 bp	1.0	79.4
rpoB-trnC-GCA_p2	TCCACGAACCTACAAAAGCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.1	58.1	1352-1359 bp	1.0	79.3
rpoB-trnC-GCA_p3	CCACGAACCTACAAAAGCCT	TTATTGATCAGGCGACACCC	50.0	50.0	58.1	57.7	1369-1376 bp	1.0	77.4
rpoB-trnC-GCA_p4	TCCACGAACCTACAAAAGCC	TTATTGATCAGGCGACACCC	50.0	50.0	58.1	57.7	1370-1377 bp	1.0	77.4
rpoB-trnC-GCA_p5	CCACGAACCTACAAAAGCCT	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	58.4	1333-1340 bp	1.0	77.2