Hotspot

ycf1

IRb · 103953-108704 · 4752 bp

Back to Passiflora

Pi 0.0184

MarkerSeek score 54.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 4752
variable_sites: 435
pi: 0.0184
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 54.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCAGAAAACCCTTACCCAA	TGCCATGCCTTCTCTGTTTT	50.0	45.0	58.0	58.0	1301 bp	0.095238	44.0
ycf1_p2	GGCAGAAAACCCTTACCCAA	CGATAACGGTGCGCTGTATA	50.0	50.0	58.0	58.0	907 bp	0.095238	43.7
ycf1_p3	GGCAGAAAACCCTTACCCAA	CAAGCTTACTTGTCTTGCGC	50.0	50.0	58.0	58.0	1271 bp	0.095238	43.7
ycf1_p4	ACGAACGTCTTGCAACTCTT	TGCCATGCCTTCTCTGTTTT	45.0	45.0	58.1	58.0	1027 bp	0.095238	43.5
ycf1_p5	AACACTGGAATCGCCCATTT	TGCCATGCCTTCTCTGTTTT	45.0	45.0	58.1	58.0	994 bp	0.095238	43.4