Hotspot

clpP

LSC · 26943-27641 · 699 bp

Back to Passiflora

Pi 0.0964

MarkerSeek score 81.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 699
variable_sites: 288
pi: 0.0964
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GCGGAAGAGGGCTTTATGAA	TGACCCTGTTAGTCCGTTCT	50.0	50.0	58.0	58.0	1646-1790 bp	1.0	91.6
clpP_p2	TGCAGTTCGATTCTGGACAG	TGACCCTGTTAGTCCGTTCT	50.0	50.0	57.9	58.0	1589-1709 bp	1.0	91.3
clpP_p3	GCGGAAGAGGGCTTTATGAA	CGAGTACATATTCCTCGGCG	50.0	55.0	58.0	58.0	1357-1444 bp	0.952381	89.5
clpP_p4	ATTTGGGTTCTGGGGTTGAC	TGACCCTGTTAGTCCGTTCT	50.0	50.0	58.0	58.0	1518-1605 bp	0.809524	84.3
clpP_p5	ATTTGGGTTCTGGGGTTGAC	CGAGTACATATTCCTCGGCG	50.0	55.0	58.0	58.0	1172-1259 bp	0.809524	84.0