Hotspot

accD

LSC · 39282-41738 · 2457 bp

Back to Passiflora

Pi 0.135

MarkerSeek score 78.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 2457
variable_sites: 1253
pi: 0.135
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAGTTAGGACTCCGCTGAAT	ACTATCCATCCGCTTGTTACT	45.0	42.9	56.3	56.5	2650-2892 bp	0.619048	43.0
accD_p2	AGTTAGGACTCCGCTGAATT	ACTATCCATCCGCTTGTTACT	45.0	42.9	56.3	56.5	2649-2891 bp	0.619048	43.0
accD_p3	AGTTAGGACTCCGCTGAATT	TCGAATAACTATCCATCCGCT	45.0	42.9	56.3	56.4	2656-2898 bp	0.619048	42.1
accD_p4	TTTCTTCTTATGCCACCCCT	ACTATCCATCCGCTTGTTACT	45.0	42.9	56.4	56.5	2928-2948 bp	0.095238	17.5
accD_p5	TTTCTTCTTATGCCACCCCT	TCGAATAACTATCCATCCGCT	45.0	42.9	56.4	56.4	2935-2955 bp	0.095238	16.6