Hotspot

trnC-GCA-psbB

LSC · 72886-73757 · 872 bp

Back to Passiflora

Pi 0.0514

MarkerSeek score 86.00

Primer available yes

Label trnC-GCA-psbB

Feature Metadata

label_name: trnC-GCA-psbB
feature_type: igs
region: LSC
length_bp: 872
variable_sites: 235
pi: 0.0514
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-psbB_p1	CGACACCCAAGATTCGAACT	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	746-1965 bp	1.0	89.5
trnC-GCA-psbB_p2	AAGATTCGAACTGGGGAACG	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	738-1957 bp	1.0	89.3
trnC-GCA-psbB_p3	ACGAGGATTTGCAGTCCTTC	CGATACCAAGGCAAACCCAT	50.0	50.0	57.9	58.2	721-1940 bp	1.0	89.3
trnC-GCA-psbB_p4	ACACCCAAGATTCGAACTGG	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	744-1963 bp	1.0	88.6
trnC-GCA-psbB_p5	AACTGGGGAACGAGGATTTG	CGATACCAAGGCAAACCCAT	50.0	50.0	57.8	58.2	855-1949 bp	0.952381	86.6