Hotspot

trnY-GUA-trnD-GUC

LSC · 69245-69755 · 511 bp

Back to Passiflora

Pi 0.0278

MarkerSeek score 83.90

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 99
pi: 0.0278
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	562-607 bp	1.0	85.4
trnY-GUA-trnD-GUC_p2	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	561-606 bp	1.0	85.2
trnY-GUA-trnD-GUC_p3	GGGGACGGACTGTAAATTCG	TTGAATACGTCGGGACTGAC	55.0	50.0	58.4	57.4	640-661 bp	0.952381	82.1
trnY-GUA-trnD-GUC_p4	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.2	58.5	563-608 bp	1.0	82.0
trnY-GUA-trnD-GUC_p5	GGGACGGACTGTAAATTCGT	TTGAATACGTCGGGACTGAC	50.0	50.0	57.6	57.4	639-660 bp	0.952381	82.0