Hotspot

trnT-GGU-trnE-UUC

LSC · 68204-69028 · 825 bp

Back to Passiflora

Pi 0.0423

MarkerSeek score 82.70

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 166
pi: 0.0423
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	CGTTGCCTCCTTGAAAGAGA	50.0	50.0	58.0	57.8	587-971 bp	1.0	90.6
trnT-GGU-trnE-UUC_p2	TACCGCTGAGTTAAAAGGGC	CGTTGCCTCCTTGAAAGAGA	50.0	50.0	57.9	57.8	552-936 bp	1.0	89.8
trnT-GGU-trnE-UUC_p3	CCGATGACTTACGCCTTACC	CGTTGCCTCCTTGAAAGAGA	55.0	50.0	58.2	57.8	584-968 bp	1.0	89.4
trnT-GGU-trnE-UUC_p4	GAACCGATGACTTACGCCTT	TCGTAATACCCTACCCCCAG	50.0	55.0	58.0	57.6	622-1006 bp	1.0	88.9
trnT-GGU-trnE-UUC_p5	GAACCGATGACTTACGCCTT	ATCCCCGATCCACTTCCTTT	50.0	50.0	58.0	58.4	641-1025 bp	0.904762	84.9