Hotspot

ndhG-ndhI

SSC · 113734-113931 · 198 bp

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Pi 0.0135

MarkerSeek score 65.90

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 198
variable_sites: 17
pi: 0.0135
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CCCAGCGAAAAGGCAGAATA	TTGAGCCGGTTACCAATCTC	50.0	50.0	58.2	57.6	375-437 bp	1.0	83.1
ndhG-ndhI_p2	CCCAGCGAAAAGGCAGAATA	AGCCGGTTACCAATCTCCAT	50.0	50.0	58.2	58.8	372-434 bp	1.0	80.0
ndhG-ndhI_p3	CCCAGCGAAAAGGCAGAATA	GCCGGTTACCAATCTCCATA	50.0	50.0	58.2	57.1	371-433 bp	1.0	78.9
ndhG-ndhI_p4	CCCAGCGAAAAGGCAGAATA	GCCGGTTACCAATCTCCATAA	50.0	47.6	58.2	57.8	371-433 bp	1.0	76.7
ndhG-ndhI_p5	CCCAGCGAAAAGGCAGAATA	AGCCGGTTACCAATCTCCA	50.0	52.6	58.2	58.3	372-434 bp	1.0	75.8