Hotspot

ccsA-ndhD

SSC · 110134-110324 · 191 bp

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Pi 0.0236

MarkerSeek score 66.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 191
variable_sites: 32
pi: 0.0236
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGTTTACATAGTTATGGTTCA	CTCTCGCTATCCGTTGACAG	31.8	55.0	52.5	57.9	286-295 bp	1.0	51.0
ccsA-ndhD_p2	AGGTTTACATAGTTATGGTTCA	TCTCTCGCTATCCGTTGACA	31.8	50.0	52.5	58.3	287-296 bp	1.0	51.0
ccsA-ndhD_p3	AGGAATAGGTTTACATAGTTATGGT	CTCTCGCTATCCGTTGACAG	32.0	55.0	55.3	57.9	292-301 bp	1.0	51.0
ccsA-ndhD_p4	AGGTTTACATAGTTATGGTTCAT	CTCTCGCTATCCGTTGACAG	30.4	55.0	53.2	57.9	286-295 bp	1.0	51.0
ccsA-ndhD_p5	AGGTTTACATAGTTATGGTTCA	CCGTTGACAGGGTAGAGACT	31.8	55.0	52.5	58.5	276-281 bp	0.956522	48.9