Hotspot

rpl32-trnL-UAG

SSC · 108472-109007 · 536 bp

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Pi 0.0223

MarkerSeek score 67.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 536
variable_sites: 69
pi: 0.0223
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ATGGGGGCTTATCCTATCCC	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	58.0	57.9	972-1002 bp	0.826087	81.9
rpl32-trnL-UAG_p2	ATGGGGGCTTATCCTATCCC	AGCAGCGTGTCTACCAATTT	55.0	45.0	58.0	57.8	965-995 bp	0.826087	81.5
rpl32-trnL-UAG_p3	ATGGGGGCTTATCCTATCCC	ATGTCCCGAAGAAACCCAAC	55.0	50.0	58.0	58.1	1285-1315 bp	0.826087	80.4
rpl32-trnL-UAG_p4	ATGGGGGCTTATCCTATCCC	CCGAAGAAACCCAACGAGAA	55.0	50.0	58.0	58.1	1280-1310 bp	0.826087	80.2
rpl32-trnL-UAG_p5	ATGGGGGCTTATCCTATCCC	GCAGATAATAAGGAGCCGCA	55.0	50.0	58.0	57.8	1563-1593 bp	0.826087	78.7