Hotspot

rbcL-psaI

LSC · 57935-58876 · 942 bp

Back to Paspalum

Pi 0.018

MarkerSeek score 70.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 942
variable_sites: 89
pi: 0.018
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	GGCTTCAGGGGGTATTCATG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1273-1353 bp	1.0	89.7
rbcL-psaI_p2	TCTCATTATTGCCGCGACAA	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	1563-1643 bp	1.0	89.0
rbcL-psaI_p3	TTGTCTCATTATTGCCGCGA	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	1566-1646 bp	1.0	88.9
rbcL-psaI_p4	GGCTTCAGGGGGTATTCATG	AATCGGTCATAACCACTGCC	55.0	50.0	58.0	58.0	1941-2027 bp	0.956522	87.0
rbcL-psaI_p5	TCTCATTATTGCCGCGACAA	AATCGGTCATAACCACTGCC	45.0	50.0	58.0	58.0	2231-2317 bp	0.956522	86.5