Hotspot

trnC-GCA-rpoB

LSC · 20123-21346 · 1224 bp

Back to Paspalum

Pi 0.0163
MarkerSeek score 76.80
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1224
variable_sites
107
pi
0.0163
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1264-1325 bp 1.0 87.4
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1263-1324 bp 1.0 87.4
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG AGTTCTTCTGCCAAGCCTTG 50.0 50.0 58.1 58.4 1327-1388 bp 1.0 86.9
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG AAGTTCTTCTGCCAAGCCTT 50.0 45.0 58.1 57.6 1328-1387 bp 0.956522 84.9
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG CAAGTTCTTCTGCCAAGCCT 50.0 50.0 58.1 58.4 1329-1388 bp 0.956522 84.7