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psbM-petN

LSC · 18213-19031 · 819 bp

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Pi 0.0113

MarkerSeek score 68.70

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 819
variable_sites: 64
pi: 0.0113
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	AAAACAGCCAGCCAAAATGA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.7	58.2	881-929 bp	1.0	78.2
psbM-petN_p2	AAAACAGCCAGCCAAAATGA	GGCTGCTTTAATGGTGGTCT	40.0	50.0	56.7	58.2	865-913 bp	1.0	78.2
psbM-petN_p3	AAAACAGCCAGCCAAAATGA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	56.7	58.3	825-873 bp	1.0	77.5
psbM-petN_p4	AACAGCCAGCCAAAATGATT	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	56.5	58.2	879-927 bp	1.0	76.3
psbM-petN_p5	AAAACAGCCAGCCAAAATGA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	56.7	58.4	824-872 bp	0.956522	74.7