Hotspot

ycf1

SSC · 130406-136078 · 5673 bp

Back to Parthenocissus

Pi 0.00764

MarkerSeek score 55.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5673
variable_sites: 65
pi: 0.00764
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCACAAATTCTGGGAAGAACC	CCAAGACTGGGTCGAATAAT	47.6	45.0	57.7	54.8	183 bp	1.0	48.7
ycf1_p2	GCACAAATTCTGGGAAGAACC	CCAAGACTGGGTCGAATAATT	47.6	42.9	57.7	55.7	183 bp	1.0	47.3
ycf1_p3	TGGTATAACCCACGGTTTCA	CCAAGACTGGGTCGAATAAT	45.0	45.0	56.5	54.8	224 bp	1.0	45.9
ycf1_p4	TGGTATAACCCACGGTTTCA	CCAAGACTGGGTCGAATAATT	45.0	42.9	56.5	55.7	224 bp	1.0	44.6
ycf1_p5	TGATTGGTATAACCCACGGT	CCAAGACTGGGTCGAATAAT	45.0	45.0	56.2	54.8	228 bp	1.0	44.0