Pi
0.000898
MarkerSeek score
59.30
Primer available
yes
Label
rpl2
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rpl2_p1 | ACGTGTCACAGTTAATTACTCCT | CGCTTCAACCGGGTTATTCT | 39.1 | 50.0 | 57.5 | 58.3 | 1631-1634 bp | 1.0 | 50.4 |
| rpl2_p2 | ACGTGTCACAGTTAATTACTCCT | ATCATTACGCTTCAACCGGG | 39.1 | 50.0 | 57.5 | 58.3 | 1638-1641 bp | 1.0 | 49.8 |
| rpl2_p3 | ACGTGTCACAGTTAATTACTCCT | ACGCTTCAACCGGGTTATTC | 39.1 | 50.0 | 57.5 | 58.6 | 1632-1635 bp | 1.0 | 48.1 |
| rpl2_p4 | ACGTGTCACAGTTAATTACTCC | CGCTTCAACCGGGTTATTCT | 40.9 | 50.0 | 56.1 | 58.3 | 1631-1634 bp | 1.0 | 47.3 |
| rpl2_p5 | ACGTGTCACAGTTAATTACTCC | ATCATTACGCTTCAACCGGG | 40.9 | 50.0 | 56.1 | 58.3 | 1638-1641 bp | 1.0 | 46.7 |
| rpl2_p1 | CGCTTCAACCGGGTTATTCT | ACGTGTCACAGTTAATTACTCCT | 50.0 | 39.1 | 58.3 | 57.5 | 1631-1634 bp | 1.0 | 50.4 |
| rpl2_p2 | ATCATTACGCTTCAACCGGG | ACGTGTCACAGTTAATTACTCCT | 50.0 | 39.1 | 58.3 | 57.5 | 1638-1641 bp | 1.0 | 49.8 |
| rpl2_p3 | ACGCTTCAACCGGGTTATTC | ACGTGTCACAGTTAATTACTCCT | 50.0 | 39.1 | 58.6 | 57.5 | 1632-1635 bp | 1.0 | 48.1 |
| rpl2_p4 | CGCTTCAACCGGGTTATTCT | ACGTGTCACAGTTAATTACTCC | 50.0 | 40.9 | 58.3 | 56.1 | 1631-1634 bp | 1.0 | 47.3 |
| rpl2_p5 | ATCATTACGCTTCAACCGGG | ACGTGTCACAGTTAATTACTCC | 50.0 | 40.9 | 58.3 | 56.1 | 1638-1641 bp | 1.0 | 46.7 |