Hotspot

rps15-ycf1

SSC · 129990-130405 · 416 bp

Back to Parthenocissus

Pi 0.0129

MarkerSeek score 58.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 416
variable_sites: 8
pi: 0.0129
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAACAGATCCCCTATTTGCT	CCACGATTGAAAACTCGTTGA	42.9	42.9	56.7	57.1	498-499 bp	1.0	48.5
rps15-ycf1_p2	TCAACAGATCCCCTATTTGCT	TCCACGATTGAAAACTCGTTG	42.9	42.9	56.7	57.1	499-500 bp	1.0	48.5
rps15-ycf1_p3	TCAACAGATCCCCTATTTGCTT	CCACGATTGAAAACTCGTTGA	40.9	42.9	57.4	57.1	498-499 bp	1.0	46.2
rps15-ycf1_p4	TCAACAGATCCCCTATTTGCTT	TCCACGATTGAAAACTCGTTG	40.9	42.9	57.4	57.1	499-500 bp	1.0	46.2
rps15-ycf1_p5	TTCAACAGATCCCCTATTTGCT	CCACGATTGAAAACTCGTTGA	40.9	42.9	57.4	57.1	499-500 bp	1.0	46.2