Hotspot

rpl32-trnL-UAG

SSC · 119478-120637 · 1160 bp

Back to Parthenocissus

Pi 0.0103
MarkerSeek score 38.50
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1160
variable_sites
18
pi
0.0103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GAAAAGGAAGGGATGGTGGG CGGGATGGAAATCGGGAATT 55.0 50.0 58.1 58.0 1425-1428 bp 1.0 79.8
rpl32-trnL-UAG_p2 GGAAAAGGAAGGGATGGTGG CGGGATGGAAATCGGGAATT 55.0 50.0 58.1 58.0 1426-1429 bp 1.0 79.8
rpl32-trnL-UAG_p3 GAAAAGGAAGGGATGGTGGG GCTGCATAGCCCAATACCAT 55.0 50.0 58.1 58.1 1921-1924 bp 1.0 79.1
rpl32-trnL-UAG_p4 GGAAAAGGAAGGGATGGTGG GCTGCATAGCCCAATACCAT 55.0 50.0 58.1 58.1 1922-1925 bp 1.0 79.1
rpl32-trnL-UAG_p5 GAAAAGGAAGGGATGGTGGG AGAAGATCCCGGGATGGAAA 55.0 50.0 58.1 58.1 1434-1437 bp 1.0 79.1