Hotspot

psaJ-rpl33

LSC · 72174-72623 · 450 bp

Back to Parthenocissus

Pi 0.0164
MarkerSeek score 63.80
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
450
variable_sites
11
pi
0.0164
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 ACATATCTTTCCACGGCACC GGCATCTTTACTCTTGGCCA 50.0 50.0 58.0 57.9 585-595 bp 1.0 80.8
psaJ-rpl33_p2 ACATATCTTTCCACGGCACC AACACCGTCTCGTACACAAC 50.0 50.0 58.0 58.2 633-643 bp 1.0 80.5
psaJ-rpl33_p3 TATGGTTCGGGTCCTTAGCA GGCATCTTTACTCTTGGCCA 50.0 50.0 58.1 57.9 551-561 bp 1.0 80.2
psaJ-rpl33_p4 TATGGTTCGGGTCCTTAGCA AACACCGTCTCGTACACAAC 50.0 50.0 58.1 58.2 599-609 bp 1.0 79.8
psaJ-rpl33_p5 CGGGTCCTTAGCAGGTCTAT GGCATCTTTACTCTTGGCCA 55.0 50.0 58.3 57.9 544-554 bp 1.0 79.0