Hotspot

ycf3

LSC · 42708-42741 · 34 bp

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Pi 0.118

MarkerSeek score 72.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 34
variable_sites: 4
pi: 0.118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TATTCCAAATCACGCGAGCA	TTCTGAAATTGCGGAGGCTT	45.0	45.0	58.0	58.1	503 bp	1.0	84.4
ycf3_p2	CGCGAGCAGACATTAGCTAT	TTCTGAAATTGCGGAGGCTT	50.0	45.0	57.9	58.1	491 bp	1.0	84.2
ycf3_p3	TATTCCAAATCACGCGAGCA	ATTCTGAAATTGCGGAGGCT	45.0	45.0	58.0	57.9	504 bp	1.0	84.0
ycf3_p4	TATTCCAAATCACGCGAGCA	GCCAGGGGGATTCTGAAATT	45.0	50.0	58.0	57.8	513 bp	1.0	83.7
ycf3_p5	ACACATACAAAGAGACCCGC	TTCTGAAATTGCGGAGGCTT	50.0	45.0	57.9	58.1	853 bp	1.0	82.3