Hotspot

ndhF-trnN

SSC · 127098-128655 · 1558 bp

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Pi 0.0066
MarkerSeek score 42.50
Primer available yes
Label ndhF-trnN

Feature Metadata

label_name
ndhF-trnN
feature_type
igs
region
SSC
length_bp
1558
variable_sites
6
pi
0.0066
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.58
markerseek_score
42.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnN_p1 TGTGGGGGTGAGAAAATTGG GGCAGAATAGCAGAGCAAGT 50.0 50.0 58.0 58.0 1425-2074 bp 1.0 84.3
ndhF-trnN_p2 TGTGGGGGTGAGAAAATTGG GTCCCCCATCAGCGTATTTT 50.0 50.0 58.0 57.9 1485-2134 bp 1.0 84.2
ndhF-trnN_p3 TGTGGGGGTGAGAAAATTGG GAACTTGGGTGTGGGTCTTT 50.0 50.0 58.0 57.9 1575-2224 bp 1.0 84.2
ndhF-trnN_p4 TGTGGGGGTGAGAAAATTGG AACGCGTTCCTCGTCATTAA 50.0 45.0 58.0 57.9 1382-2031 bp 1.0 84.1
ndhF-trnN_p5 GGGGGTGGACGTATTTCTTC GGCAGAATAGCAGAGCAAGT 55.0 50.0 58.0 58.0 1307-1956 bp 1.0 83.9