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clpP-psbB

LSC · 72351-72849 · 499 bp

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Pi 0.0343

MarkerSeek score 74.20

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 499
variable_sites: 16
pi: 0.0343
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	TCCTCTCCAGGACTTCGAAA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	535-566 bp	1.0	82.0
clpP-psbB_p2	TCCTCTCCAGGACTTCGAAA	AGCAACCAAAGCTGTATGCA	50.0	45.0	57.7	58.4	608-639 bp	1.0	80.6
clpP-psbB_p3	TCCTCTCCAGGACTTCGAAA	ACACGATACCAAGGCAAACC	50.0	50.0	57.7	58.5	538-569 bp	1.0	80.1
clpP-psbB_p4	GATGCATCTTCCTCTCCAGG	CGATACCAAGGCAAACCCAT	55.0	50.0	57.5	58.2	544-575 bp	1.0	80.0
clpP-psbB_p5	TCCTCTCCAGGACTTCGAAA	TGAACACGATACCAAGGCAA	50.0	45.0	57.7	57.4	541-572 bp	1.0	79.4