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psbL-psbF

LSC · 64588-64609 · 22 bp

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Pi 0.0455
MarkerSeek score 73.70
Primer available yes
Label psbL-psbF

Feature Metadata

label_name
psbL-psbF
feature_type
igs
region
LSC
length_bp
22
variable_sites
1
pi
0.0455
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbL-psbF_p1 ATAACCCCCAGTAGAGGCTG GCCTAGCTGTACCTACCGTT 55.0 55.0 58.2 58.9 154 bp 1.0 71.9
psbL-psbF_p2 ATAACCCCCAGTAGAGGCTG GGGGTCAATATCAGCAATGC 55.0 50.0 58.2 56.9 126 bp 1.0 70.3
psbL-psbF_p3 ATAACCCCCAGTAGAGGCTG TTTACAGTGCGATGGTTGGC 55.0 50.0 58.2 59.1 182 bp 1.0 69.9
psbL-psbF_p4 ATAACCCCCAGTAGAGGCTG GCCTAGCTGTACCTACCGT 55.0 57.9 58.2 58.2 154 bp 1.0 69.4
psbL-psbF_p5 AATAACCCCCAGTAGAGGCT GCCTAGCTGTACCTACCGTT 50.0 55.0 57.4 58.9 155 bp 1.0 68.7