Hotspot

ndhC-atpE

LSC · 50544-52634 · 2091 bp

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Pi 0.0295

MarkerSeek score 62.90

Primer available yes

Label ndhC-atpE

Feature Metadata

label_name: ndhC-atpE
feature_type: igs
region: LSC
length_bp: 2091
variable_sites: 61
pi: 0.0295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-atpE_p1	TCATATTCGTAAAGCAGAAACA	CAAATCTAGCTCTCCGACGG	31.8	55.0	53.9	57.9	2173-2219 bp	1.0	43.0
ndhC-atpE_p2	TCATATTCGTAAAGCAGAAACA	GGCAAATCTAGCTCTCCGAC	31.8	55.0	53.9	58.1	2175-2221 bp	1.0	43.0
ndhC-atpE_p3	TCATATTCGTAAAGCAGAAACA	CGAGGCAAATCTAGCTCTCC	31.8	55.0	53.9	57.9	2178-2224 bp	1.0	43.0
ndhC-atpE_p4	TCATATTCGTAAAGCAGAAACA	GAGGCAAATCTAGCTCTCCG	31.8	55.0	53.9	57.9	2177-2223 bp	1.0	43.0
ndhC-atpE_p5	TCATATTCGTAAAGCAGAAACA	AGGCAAATCTAGCTCTCCGA	31.8	50.0	53.9	58.2	2176-2222 bp	1.0	43.0