Hotspot

psbC-trnS-UGA

LSC · 35379-35642 · 264 bp

Back to Parthenium

Pi 0.0717

MarkerSeek score 74.00

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 264
variable_sites: 18
pi: 0.0717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCATGACCCCGCTTAATTGA	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.9	58.0	353-366 bp	1.0	86.5
psbC-trnS-UGA_p2	TCCATGACCCCGCTTAATTG	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.9	58.0	354-367 bp	1.0	86.5
psbC-trnS-UGA_p3	GGTGGAGTAGCTACCGAGAT	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.0	58.0	534-547 bp	1.0	84.4
psbC-trnS-UGA_p4	GGTGGAGTAGCTACCGAGAT	GTCTTTCGGAGAGATGGCTG	55.0	55.0	58.0	58.1	542-555 bp	1.0	84.0
psbC-trnS-UGA_p5	TCTAAGGGGTCCAAATGGGT	GAGAGATGGCTGAGTGGTTG	50.0	55.0	57.9	58.0	652-665 bp	1.0	83.3