Hotspot

atpl-atpH

LSC · 25121-26276 · 1156 bp

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Pi 0.032

MarkerSeek score 70.90

Primer available yes

Label atpl-atpH

Feature Metadata

label_name: atpl-atpH
feature_type: igs
region: LSC
length_bp: 1156
variable_sites: 37
pi: 0.032
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpl-atpH_p1	CCCGTCATGTTCCTTGGATT	ATAACGGAAGCGGCAGAAAT	50.0	45.0	57.9	58.0	1290-1338 bp	1.0	81.0
atpl-atpH_p2	CCCGTCATGTTCCTTGGATT	TACCTTGACCAACTCCAGGT	50.0	50.0	57.9	57.9	1346-1394 bp	1.0	80.3
atpl-atpH_p3	CCCGTCATGTTCCTTGGATT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.9	57.9	1306-1354 bp	1.0	80.1
atpl-atpH_p4	CCCGTCATGTTCCTTGGATT	AATAACGGAAGCGGCAGAAA	50.0	45.0	57.9	58.2	1291-1339 bp	1.0	79.8
atpl-atpH_p5	CCCGTCATGTTCCTTGGATT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.9	58.2	1350-1398 bp	1.0	79.7