Hotspot

psbM-rpoB

LSC · 10884-12900 · 2017 bp

Back to Parthenium

Pi 0.188

MarkerSeek score 91.50

Primer available yes

Label psbM-rpoB

Feature Metadata

label_name: psbM-rpoB
feature_type: igs
region: LSC
length_bp: 2017
variable_sites: 337
pi: 0.188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 91.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-rpoB_p1	TTGACTGGCTGTTTTTACGT	CTTCTGCCAAACCCTGATCA	40.0	50.0	55.8	57.8	2189-2251 bp	1.0	72.9
psbM-rpoB_p2	TTTGACTGGCTGTTTTTACGT	CTTCTGCCAAACCCTGATCA	38.1	50.0	56.6	57.8	2190-2252 bp	1.0	71.1
psbM-rpoB_p3	TTGACTGGCTGTTTTTACGT	TCTTCTGCCAAACCCTGATC	40.0	50.0	55.8	57.5	2190-2252 bp	1.0	70.6
psbM-rpoB_p4	TTGACTGGCTGTTTTTACGT	CTCTTCTGCCAAACCCTGAT	40.0	50.0	55.8	57.5	2191-2253 bp	1.0	70.5
psbM-rpoB_p5	TTGACTGGCTGTTTTTACGT	TGCCAAACCCTGATCAATGA	40.0	45.0	55.8	57.4	2185-2247 bp	1.0	69.7