Hotspot

matK-rps16

LSC · 3543-4961 · 1419 bp

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Pi 0.0353

MarkerSeek score 64.80

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: LSC
length_bp: 1419
variable_sites: 50
pi: 0.0353
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	GTTGTGCCCCAAAAATGGAG	CCCAGAACAAGGAAACACCA	50.0	50.0	57.8	57.9	2264-2288 bp	1.0	81.0
matK-rps16_p2	TTGTGCCCCAAAAATGGAGT	CCCAGAACAAGGAAACACCA	45.0	50.0	58.2	57.9	2263-2287 bp	1.0	80.7
matK-rps16_p3	GTTGTGCCCCAAAAATGGAG	GCCGTACGAGGAGAAAACTT	50.0	50.0	57.8	57.9	1965-1994 bp	1.0	80.6
matK-rps16_p4	TTGTGCCCCAAAAATGGAGT	GCCGTACGAGGAGAAAACTT	45.0	50.0	58.2	57.9	1964-1993 bp	1.0	80.4
matK-rps16_p5	GTTGTGCCCCAAAAATGGAG	AGCCGTCTATCGAATCGTTG	50.0	50.0	57.8	57.9	1897-1925 bp	1.0	80.3