Hotspot

psbA-matK

LSC · 1468-2027 · 560 bp

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Pi 0.0537
MarkerSeek score 73.20
Primer available yes
Label psbA-matK

Feature Metadata

label_name
psbA-matK
feature_type
igs
region
LSC
length_bp
560
variable_sites
30
pi
0.0537
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-matK_p1 CAGACGGAAACTAAGCTCCC GAGCGCTTTGGCCGTATATA 55.0 50.0 58.0 57.9 1283-1298 bp 1.0 81.8
psbA-matK_p2 CCAGACGGAAACTAAGCTCC GAGCGCTTTGGCCGTATATA 55.0 50.0 58.0 57.9 1284-1299 bp 1.0 81.8
psbA-matK_p3 CCCAGACGGAAACTAAGCTC GAGCGCTTTGGCCGTATATA 55.0 50.0 58.0 57.9 1285-1300 bp 1.0 81.8
psbA-matK_p4 CGGAAACTAAGCTCCCACTC GAGCGCTTTGGCCGTATATA 55.0 50.0 58.0 57.9 1279-1294 bp 1.0 81.8
psbA-matK_p5 CGGAAACTAAGCTCCCACTC GTACGCGGTCAAATGCTAGA 55.0 50.0 58.0 58.1 1456-1471 bp 1.0 81.8