Hotspot

ndhI

SSC · 119565-120068 · 504 bp

Pi 0.444

MarkerSeek score 77.40

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 504
variable_sites: 179
pi: 0.444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	ATTTGTGGCGTCAACCGATA	GTGCAGCAGCTACAAAATGG	45.0	50.0	57.9	58.0	1554 bp	0.5	59.1
ndhI_p2	ATTTGTGGCGTCAACCGATA	CCATTCCCAACGGTCCATAG	45.0	55.0	57.9	58.0	1663 bp	0.5	58.9
ndhI_p3	ATTTGTGGCGTCAACCGATA	GCCCCCACGTAAATAAGGAG	45.0	55.0	57.9	58.0	1576 bp	0.5	58.9
ndhI_p4	ATTTGTGGCGTCAACCGATA	TAACCCCGTACCATGACGTA	45.0	50.0	57.9	57.9	1740 bp	0.5	58.1
ndhI_p5	ATTTGTGGCGTCAACCGATA	GTTTATGGCCCCCACGTAAA	45.0	50.0	57.9	58.2	1583 bp	0.5	57.9