Hotspot

ndhG

SSC · 120387-120929 · 543 bp

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Pi 0.451
MarkerSeek score 72.90
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
SSC
length_bp
543
variable_sites
148
pi
0.451
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.60
markerseek_score
72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 TAACCTCTGAACGTTTCCGC TCGATTGGTTGATGCGAGTT 50.0 45.0 58.2 57.9 1749 bp 0.5 57.5
ndhG_p2 TAACCTCTGAACGTTTCCGC CGATTGGTTGATGCGAGTTG 50.0 50.0 58.2 57.8 1748 bp 0.5 57.0
ndhG_p3 TAACCTCTGAACGTTTCCGC GCGGCTGCAATTGCTATAAC 50.0 50.0 58.2 58.2 1675 bp 0.5 56.4
ndhG_p4 TAACCTCTGAACGTTTCCGC AGCGGCTGCAATTGCTATAA 50.0 45.0 58.2 58.3 1676 bp 0.5 55.7
ndhG_p5 TCTGCCTGTTGTTGATTGGA TCGATTGGTTGATGCGAGTT 45.0 45.0 57.3 57.9 1661 bp 0.5 54.0