Hotspot

ndhA

SSC · 117309-119480 · 2172 bp

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Pi 0.41
MarkerSeek score 80.60
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2172
variable_sites
794
pi
0.41
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.89
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 AGTTGAAGCTCCAAAAGGGG CAGCTCGTAGGGTTTGTTGA 50.0 50.0 58.0 57.8 2508 bp 0.5 58.7
ndhA_p2 AGTTGAAGCTCCAAAAGGGG AGCTCGTAGGGTTTGTTGAC 50.0 50.0 58.0 57.8 2507 bp 0.5 58.7
ndhA_p3 GTTGAAGCTCCAAAAGGGGA CAGCTCGTAGGGTTTGTTGA 50.0 50.0 58.0 57.8 2507 bp 0.5 58.7
ndhA_p4 GTTGAAGCTCCAAAAGGGGA AGCTCGTAGGGTTTGTTGAC 50.0 50.0 58.0 57.8 2506 bp 0.5 58.6
ndhA_p5 AGTTGAAGCTCCAAAAGGGG CCAATATACCTGGCAGCTCG 50.0 55.0 58.0 58.2 2521 bp 0.5 58.4