Hotspot

ccsA

SSC · 123838-124818 · 981 bp

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Pi 0.442
MarkerSeek score 79.70
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
981
variable_sites
351
pi
0.442
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 TTATCGATGGCTTCCCTTGC GTTTTGAACAAGTTGCCGCT 50.0 45.0 58.0 58.1 1769 bp 0.5 59.1
ccsA_p2 TTATCGATGGCTTCCCTTGC AAGTTGCCGCTATGGTGAAA 50.0 45.0 58.0 58.1 1760 bp 0.5 59.0
ccsA_p3 TTATCGATGGCTTCCCTTGC TAGACACGCTGCTCTTAGGA 50.0 50.0 58.0 57.9 1736 bp 0.5 58.7
ccsA_p4 TTATCGATGGCTTCCCTTGC AGTTGCCGCTATGGTGAAAT 50.0 45.0 58.0 57.9 1759 bp 0.5 58.7
ccsA_p5 TTATCGATGGCTTCCCTTGC AAATTGGTAGACACGCTGCT 50.0 45.0 58.0 57.8 1743 bp 0.5 58.3