Hotspot

ndhG-ndhE

SSC · 120930-121166 · 237 bp

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Pi 0.427
MarkerSeek score 77.30
Primer available yes
Label ndhG-ndhE

Feature Metadata

label_name
ndhG-ndhE
feature_type
igs
region
SSC
length_bp
237
variable_sites
96
pi
0.427
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhE_p1 CCATTTTGTAGCTGCTGCAC ACATCTCTTACAACCGACGC 50.0 50.0 58.0 58.0 1325 bp 0.5 59.8
ndhG-ndhE_p2 CCATTTTGTAGCTGCTGCAC CATCTCTTACAACCGACGCA 50.0 50.0 58.0 58.0 1324 bp 0.5 59.8
ndhG-ndhE_p3 TCTGTGGCTCGTCAACAAAA ACATCTCTTACAACCGACGC 45.0 50.0 58.0 58.0 976 bp 0.5 59.7
ndhG-ndhE_p4 TTCTGTGGCTCGTCAACAAA ACATCTCTTACAACCGACGC 45.0 50.0 58.0 58.0 977 bp 0.5 59.7
ndhG-ndhE_p5 TTTCTGTGGCTCGTCAACAA ACATCTCTTACAACCGACGC 45.0 50.0 58.0 58.0 978 bp 0.5 59.7