Hotspot

trnN-GUU-rps15

IRb · 109429-115748 · 6320 bp

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Pi 0.0366
MarkerSeek score 76.40
Primer available yes
Label trnN-GUU-rps15

Feature Metadata

label_name
trnN-GUU-rps15
feature_type
igs
region
IRb
length_bp
6320
variable_sites
224
pi
0.0366
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rps15_p1 CCCGGAACTTCTTCGTAGTG ACGAACATCATGAGCTGTCC 55.0 50.0 58.0 58.0 587-702 bp 1.0 84.5
trnN-GUU-rps15_p2 CCCGGAACTTCTTCGTAGTG GGAAGAGATAAGAGGGCCCT 55.0 55.0 58.0 57.9 484-599 bp 1.0 84.2
trnN-GUU-rps15_p3 CGTAGTGTCTCCGTTCCATG ACGAACATCATGAGCTGTCC 55.0 50.0 58.1 58.0 574-689 bp 1.0 84.0
trnN-GUU-rps15_p4 CCCGGAACTTCTTCGTAGTG CGTGTTGTTGCATCGAAACA 55.0 45.0 58.0 57.9 861-976 bp 1.0 83.9
trnN-GUU-rps15_p5 CAATTCCCGTTCTCAACCCA ACGAACATCATGAGCTGTCC 50.0 50.0 58.1 58.0 641-756 bp 1.0 83.6